de.STAIR: Structured Analysis and Integration of RNA-Seq Experiments

de.STAIR: Structured Analysis and Integration of RNA-Seq Experiments

RNA Sequencing (RNA-Seq) has become a widely used tool to study quantitative and qualitative aspects of the transcriptome. The variety of RNA-Seq protocols, experimental study designs and the characteristic properties of the organisms under investigation greatly affect downstream and comparative analyses. We provide easy access to comprehensive analysis of RNA-Seq experiments as a service. To do so, we leverage on the Galaxy framework, and organise dedicated workshops, training programs, and screencasts to make Life Scientists familiar with computational approaches to biological problems.

We provide these services as part of the German Network for Bioinformatics Infrastructure (de.NBI), which is the German node of ELIXIR. Here, we:

  • Build flexible workflows for RNA-Seq protocols and experimental designs
  • Integrate novel analysis methods with the RNA Bioinformatics Center (RBC) workbench

These new workflows and tools will become integral parts of RBC's Galaxy instance, to ensure portability of tools, workflows and pipelines, ultimately facilitating reproducibility. To make users confident with the Galaxy platform, we take part in the de.NBI training program, and contribute to the Galaxy training material. Finally, we provide video tutorials for introducing the Galaxy platform.


Training courses

We are available for on-site training courses, to cover diverse aspects of RNA-Seq topics, such as data pre-processing, mapping, and visualisation of differential gene expression analyses. Contact us!

October 2016 Berlin Using NGS Data Analysis in the Clinic
December 2016 Porto Genomic Applications in Biotechnology
March 2017 Ljubljana Customized Sequencing Data Analysis with Galaxy
September 2017 Oldenburg Introduction to RNA-Seq Data Analysis with Galaxy
October 2017 Freiburg A Primer for RNA-Seq Processing, Interpreting and Visualization
March 2018 Kiel Introduction to RNA-Seq Data Analysis with Galaxy
June 2018 Jena A Primer for RNA-Seq Processing, Interpretation and Visualization
September 2018 Osnabrück
RNA-Seq data analysis with Galaxy for clinical applications
March 2019 Rostock Galaxy for linking bisulfite sequencing with RNA sequencing




Twitter: @denbi_deSTAIR

Contribute: Github



This project is funded by the German Federal Ministry for Education and Research (BMBF) program de.NBI-Partner (Grant 031L0106).

Visit our official Project-Website:

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