Stefan Pauleweit

My research is focused in the analysis of the spatial cytoskeleton forming process of osteoblasts under different conditions.

PhD Students (from 2009-01-01 until 2012-12-31)

Research interest

Cytoskeleton and Actin Polymerisation

"My research is focused in the analysis of the spatial cytoskeleton forming process of osteoblasts under different conditions."

An important aspect in the interaction of bone-building cells (osteoblasts) to an artificial implant is the structural composition of the cytosceleton of these cells. The spatial actin formation is crucial for the appropriated function of these cells and can be influenced by the nanostructured surface design of the used implant material.

For the modelling should answer the question, how a well defined geometrical nanostructure of a surface is acting to the spatial filament structure of a cell.

The tracking of the actin polymerisation using a computational model reduces the limitations of the snapshot microscopy and allows the dynamic observations to every time point. In consequence, the fine grained observation of the system response to different geometrical design and possible causes can be analysed. This may help to develop a new generation of implants.

I developed a new agent-based model for the actin polymerisation. Using the assumption, that the probability of the linkage of two actin molecules is dependent to the underlying surface design, the simulations show the spatial reproduction of the surface structure. This was also shown in experiments.

Without using expensive experimental technologies, the clustering of the actin filament structure on a geometrical surface was successful reproduced by a model. The used principals will be extended by adding other involved proteins, like Arp2/3, and high scaled simulations using a big number of molecules. The model will allow a prediction of the cell behaviour on unknown surface designs.

Academic background

2009 - 2012 PhD-Student in Systems Biology and Bioinformatics
University of Rostock, Rostock/Germany
2002 - 2008 Diplom in Computer Science (Dipl. Inf., comparable to a masters degree)
University of Rostock, Rostock/Germany
1996 - 2002 German Navy, including qualification of craft certification for communication electronics technician


Selected publications

2-DE profiling of GDNF overexpression-related proteome changes in differentiating ST14A rat progenitor cells

Hoffrogge R, Beyer S, Hübner R, Mikkat S, Mix E, Scharf C, Schmitz U, Pauleweit S, Berth M, Zbrzycki IZ, Christoph H, Pahnke J, Wolkenhauer O, Uhrmacher A, Völker U, Rolfs R (2007)

Proteomics 7: 33-46

Modelling Molecular Processes by Individual-Based Simulations Applied to Actin Polymerisation

Pauleweit S, Nebe BJ, Wolkenhauer O

in: Simulation and Modeling Methodologies, Technologies and Applications; Nuno P, Janusz K, Joaquim F (eds.); series: Advances in Intelligent Systems and Computing.

ISBN 13 (print): 9783642343353

Agent-based simulation of molecular processes

Pauleweit S, Nebe J B, Wolkenhauer O

SIMULTECH 2011, Noordwijkerhout, Netherlands, July 2011

Venue: Noordwijkerhout, Netherlands


Analysis and simulation of electrical interactions of implants with biosystems

van Rienen U, Appali R, Bader R, Baumann W, Beck U, Behrend D, Benecke R, Biala K, Birkholz H, Burkel E, Engel K, Ewald H, Gimsa J, Gimsa U, Gongadze E, Grünbaum A, Haba Y, Liese F, Liu B, Lüder M, Pauleweit S, Mittelmeier W, Mix E, Nebe JB,....[+12]

44. DGBMT Jahrestagung, Rostock, Germany, 05-08 October 2010

Venue: Rostock, Germany

Downloads & more:

see program for the full list of authors: DGBMT program

Software Supporting Collaborations

Stefan Pauleweit

Summer School on Systems Biology for Medical Applications, Tenerife, Spain, 30.09.-02.10.2008

Venue: Tenerife, Spain

Systembiologie im Welisa

Pauleweit S

Poster presentation for "Lange Nacht der Wissenschaften", 29.04.2010, University of Rostock

Downloads & more:

Simulation der Messung eines Cell Monitoring Systems (Modellbildung und Parameterschätzung)

Pauleweit S


Ansäuerungsleistung und Sauerstoffverbrauch von Säugetierzellen im extrazellulären Medium

Pauleweit S