Introduction to RNA-Seq data analysis with Galaxy


The de.NBI partner de.STAIR
Andrea Bagnacani
Markus Wolfien

March 7, 2018 - 08:30 - 16:30

Kiel, Am Botanischen Garten 1-9, Room E49

RNA-Sequencing (RNA-Seq) has become a widely used approach to study quantitative and qualitative aspects of transcriptome data. The variety of RNA-Seq protocols, experimental study designs and the obtained data processing strategies greatly affect downstream and comparative analyses. Unless the low entrance barriers and easy-to-use experimental protocols, the challenge of proper, transparent and reproducible data analyses are still a bottleneck. With respect to the number of data analysis steps, the complexity of decisions on tool selection is increasing likewise.

Learning goals:
In this training, we will give an introduction to the user-friendly and openly available Galaxy framework for scientific data analyses. We will (i) teach introductory methods for the first data exploration and quality control of NGS datasets, (ii) show concepts for genome alignment and data visualization of the subsequent results and (iii) investigate a showcase project that involves the analysis of clinical NGS expression data.


RNA-Seq, quality control, assembly, quantification, visualization

Markus Wolfien,
This course is designed for 15 participants. If we receive (a lot) more applications, we will choose participants based on the relevance of their application and/or a first-come-first-serve basis.

Deadline: The REGISTRATION closes on February 12th 2018

Location: Kiel, Am Botanischen Garten 1-9, Room E49

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